When I searched this forum, I couldn't find any way to get a pixel color from an image.
Done some research and found this on a french AutoIt forum (http://www.autoitscript.fr/forum/viewtopic.php?f=3&t=2993&start=0)
#include <GDIPlus.au3>
$sv_ImageSource = @ScriptDir & "\Source.jpg"
$iPosX = 253
$iPosY = 291
_GDIPlus_Startup()
$hImage = _GDIPlus_ImageLoadFromFile($sv_ImageSource)
MsgBox(0, "Pixel Color", _GDIPlus_BitmapGetPixel($hImage, $iPosX, $iPosY))
_GDIPlus_ImageD